Heritable Epigenomic Changes to the Maize Methylome Resulting from Tissue Culture.

ABSTRACT: DNA methylation can contribute to the maintenance of genome integrity and regulation of gene expression. In most situations, DNA methylation patterns are inherited quite stably. However, changes in DNA methylation can occur at some loci as a result of tissue culture resulting in somaclonal variation. To investigate heritable epigenetic changes as a consequence of tissue culture, a sequence-capture bisulfite sequencing approach was implemented to monitor context-specific DNA methylation patterns in ∼15 Mb of the maize genome for a population of plants that had been regenerated from tissue culture. Plants that have been regenerated from tissue culture exhibit gains and losses of DNA methylation at a subset of genomic regions. There was evidence for a high rate of homozygous changes to DNA me

SUBMITTER: Han Z 

PROVIDER: S-EPMC6063226 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature