Characterization of hepatocellular adenoma and carcinoma using microRNA profiling and targeted gene sequencing.

ABSTRACT:

Background

Hepatocellular adenomas (HCA) are benign liver tumors that may transform into hepatocellular carcinoma (HCC), but the molecular drivers of this transformation remain ill-defined. This study evaluates the molecular changes in HCA and HCC and in comparison to their adjacent non-neoplastic liver.

Methods

11 patients with HCA and 10 patients with HCC without underlying hepatitis or cirrhosis were included in this pilot study. Tumor and non-tumor liver tissues were selected for immunohistochemical staining, small RNA sequencing, and targeted gene sequencing. We compared microRNA expressions and mutations between HCA and HCC and non-neoplastic liver.

Results

HCA were classified as inflammatory (n = 6), steatotic (n = 4), or β-catenin activated (n = 1) subtypes. M

SUBMITTER: Zheng J 

PROVIDER: S-EPMC6063411 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature