Endoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats.

ABSTRACT: Owing to the recent progress in regenerative medicine technology, clinical trials that harnessed the regeneration and immune modulation potentiality of stem cells for treating IBD have shown promising results. We investigated the feasibility and utility of intraluminal endoscopic transplantation of rat MSC sheets in murine models of experimental colitis for targeted delivery of stem cells to lesions. We isolated adipose-derived mesenchymal stem cells (AD-MSC) and bone marrow-derived mesenchymal stem cells (BM-MSC) from EGFP-transgenic rats and fabricated the cells in sheet forms using temperature-responsive culture dishes. The MSC sheets were endoscopically transplanted to the inflamed area in electrocoagulation and DNBS colitis model. The effect of the transplantation was verified using e

SUBMITTER: Pak S 

PROVIDER: S-EPMC6063883 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature