Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

ABSTRACT: Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts and the encoded proteins after each passage. In parallel, we investigated the differentiation potential of ASCs into chondrocytes, osteocytes and adipocytes. We further determined the effects of FBS supplementation and subsequent deprivation on the whole transcriptome by comprehensive mRNA and miRNA sequencing. Our results show that ASCs maintain differentiati

SUBMITTER: Mieczkowska A 

PROVIDER: S-EPMC6063933 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature