Effect of transcription terminator usage on the establishment of transgene transcriptional gene silencing.

ABSTRACT:

Objective

Obtaining high and stable transgene expression is of vital importance for plant genetic engineering. A lot is known about the relationship between terminator efficiency and gene expression, but no studies have addressed the relationship between terminator usage and transgene expression stability or heritable gene silencing. In this paper, we aim to analyze if terminators are a determining factor in the establishment of promoter DNA methylation of plant transgenes.

Results

Our experiments comparing plants with a LUC reporter under the 35S CaMV promoter and good efficiency terminators (Thsp, T35S) show that the use of efficient terminator sequences does not avoid the accumulation of promoter DNA methylation and transgene silencing. However, Thsp lead to a higher repo

SUBMITTER: Perez-Gonzalez A 

PROVIDER: S-EPMC6064074 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature