A Versatile Method for Viral Transfection of Calcium Indicators in the Neonatal Mouse Brain.

ABSTRACT: The first three postnatal weeks in rodents are a time when sensory experience drives the maturation of brain circuits, an important process that is not yet well understood. Alterations in this critical period of experience-dependent circuit assembly and plasticity contribute to several neurodevelopmental disorders, such as autism, epilepsy, and schizophrenia. Therefore, techniques for recording network activity and tracing neuronal connectivity over this time period are necessary for delineating circuit refinement in typical development and how it deviates in disease. Calcium imaging with GCaMP6 and other genetically encoded indicators is rapidly becoming the preferred method for recording network activity at the single-synapse and single-cell level in vivo, especially in geneticall

SUBMITTER: He CX 

PROVIDER: S-EPMC6064716 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature