Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community.

ABSTRACT: Aim: The purpose of this study was to investigate strain dependent differences of the cariogenic biofilm forming Streptococcus mutans within both simple and complex communities. Methods: A mono-species containing representative S. mutans clinical isolates (caries and non-caries), and a multispecies in vitro caries biofilm model containing Lactobacillus casei, Veillonella dispar, Fusobacterium nucleatum and Actinomyces naeslundii, and either of two representative S. mutans clinical isolates (caries and non-caries), was developed as a comparison model. Compositional analysis of total and live bacteria within biofilms, and transcriptional analysis of biofilm associated virulence factors were evaluated by live/dead PCR and q

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC6064717 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature