The Calcium-Dependent Protease Calpain-1 Links TRPC6 Activity to Podocyte Injury.

ABSTRACT:

Background

The hallmark of podocytopathies, such as FSGS, is podocyte injury resulting in proteinuria. Transient receptor potential channel C6 (TRPC6) is a calcium-conducting ion channel expressed at the slit diaphragm. TRPC6 gain-of-function mutations and glomerular TRPC6 overexpression are associated with proteinuria. However, the pathways linking TRPC6 to podocyte injury, which is characterized by loss of the slit diaphragm protein nephrin, activation of several intracellular pathways (including calcineurin-NFAT signaling), and cytoskeletal rearrangement, remain elusive.

Methods

We tested whether the calcium-dependent protease calpain-1 mediates TRPC6-dependent podocyte injury in human and experimental FSGS and cultured podocytes.

Results

Compared with kidneys of h

SUBMITTER: Verheijden KAT 

PROVIDER: S-EPMC6065075 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature