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You won't believe this old test … that does cheap single-cell mutation detection.

ABSTRACT: Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.

SUBMITTER: Kobel M

PROVIDER: S-EPMC6065114 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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You won't believe this old test … that does cheap single-cell mutation detection.

Köbel Martin M Anglesio Michael S MS Brenton James D JD

The journal of pathology. Clinical research 20180701 3

Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context. ...[more]

PMID: 30003713

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