DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China.

ABSTRACT: Although DNA barcoding has been widely used to identify plant species composition in temperate and tropical ecosystems, relatively few studies have used DNA barcodes to document both herbaceous and woody components of forest plot. A total of 201 species (72 woody species and 129 herbaceous species) representing 135 genera distributed across 64 families of seed plants were collected in a 25 ha CForBio subalpine forest dynamics plot. In total, 491 specimens were screened for three DNA regions of the chloroplast genome (rbcL, matK, and trnH-psbA) as well as the internal transcribed spacers (ITS) of nuclear ribosomal DNA. We quantified species resolution for each barcode separately or in combination using a ML tree-based method. Amplification and sequencing success

SUBMITTER: Tan SL 

PROVIDER: S-EPMC6065341 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature