DNA barcoding of economically important freshwater fish species from north-central Nigeria uncovers cryptic diversity.

ABSTRACT: This study examines the utility of morphology and DNA barcoding in species identification of freshwater fishes from north-central Nigeria. We compared molecular data (mitochondrial cytochrome c oxidase subunit I (COI) sequences) of 136 de novo samples from 53 morphologically identified species alongside others in GenBank and BOLD databases. Using DNA sequence similarity-based (≥97% cutoff) identification technique, 50 (94.30%) and 24 (45.30%) species were identified to species level using GenBank and BOLD databases, respectively. Furthermore, we identified cases of taxonomic problems in 26 (49.00%) morphologically identified species. There were also four (7.10%) cases of mismatch in DNA barcoding in which our query sequence in GenBank and BOLD showed a sequence match with dif

SUBMITTER: Iyiola OA 

PROVIDER: S-EPMC6065348 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature