The actin capping protein in Aspergillus nidulans enhances dynein function without significantly affecting Arp1 filament assembly.

ABSTRACT: The minus-end-directed microtubule motor cytoplasmic dynein requires the dynactin complex for in vivo functions. The backbone of the vertebrate dynactin complex is the Arp1 (actin-related protein 1) mini-filament whose barbed end binds to the heterodimeric actin capping protein. However, it is unclear whether the capping protein is a dynactin component in lower eukaryotic organisms, especially because it does not appear to be a component of the budding yeast dynactin complex. Here our biochemical data show that the capping protein is a component of the dynactin complex in the filamentous fungus Aspergillus nidulans. Moreover, deletion of the gene encoding capping protein alpha (capA) results in a defect in both nuclear distribution and early-endosome transport, two dynein-mediated processe

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6065395 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature