A Sarcoptes scabiei specific isothermal amplification assay for detection of this important ectoparasite of wombats and other animals.

ABSTRACT:

Background

The globally distributed epidermal ectoparasite, Sarcoptes scabiei, is a serious health and welfare burden to at-risk human and animal populations. Rapid and sensitive detection of S. scabiei infestation is critical for intervention strategies. While direct microscopy of skin scrapings is a widely utilised diagnostic method, it has low sensitivity. PCR, alternatively, has been shown to readily detect mite DNA even in microscopy-negative skin scrapings. However, a limitation to the latter method is the requirements for specialised equipment and reagents. Such resources may not be readily available in regional or remote clinical settings and are an important consideration in diagnosis of this parasitic disease.

Methodology

A Loop Mediated Isothermal Am

SUBMITTER: Fraser TA 

PROVIDER: S-EPMC6065476 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature