S1 gene sequence analysis of new variant isolates of avian infectious bronchitis virus in Tunisia.

ABSTRACT:

Purpose

Tissue samples were collected from suspected broiler flocks showing respiratory signs to identify infectious bronchitis virus (IBV), characterize emerging field strains, and study their relationships with the Massachusetts H120 strain, the only IB vaccine used in Tunisia.

Samples and methods

Several IBV isolates were identified from field samples collected from flocks located in different regions in the northeast of Tunisia. The IBV isolates were characterized and compared to commonly used vaccine strains (including 793B, D274, and H120 types), other reference IBV strains from Europe, and the recently characterized Tunisian field variants TN20/00, TN200/01, and TN335/01. Reverse transcription-polymerase chain reaction and nucleotide sequencing analyses of the hyperva

SUBMITTER: Bourogaa H 

PROVIDER: S-EPMC6065601 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature