Study of purinosome assembly in cell-based model systems with de novo purine synthesis and salvage pathway deficiencies.

ABSTRACT:

Background

The enzymes involved in de novo purine synthesis (DNPS), one of the basic processes in eukaryotic cells, transiently and reversibly form a dynamic multienzyme complex called the purinosome in the cytoplasm. The purinosome has been observed in a broad spectrum of cells, but some studies claim that it is an artefact of the constructs used for visualization or stress granules resulting from the exposure of cells to nutrient-reduced growth media. Both may be true depending on the method of observation. To clarify this point, we combined two previously used methods, transfection and immunofluorescence, to detect purinosomes in purinosome-free cells deficient in particular DNPS steps (CR-DNPS cells) and in cells deficient in the salvage pathway, which resulted in construction

SUBMITTER: Baresova V 

PROVIDER: S-EPMC6066232 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature