Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression.

ABSTRACT:

Purpose

Variants of complement factor genes, hypoxia and oxidative stress of the outer retina, and systemic hypertension affect the risk of age-related macular degeneration. Hypertension often results from the high intake of dietary salt that increases extracellular osmolarity. We determined the effects of extracellular hyperosmolarity, hypoxia, and oxidative stress on the expression of complement genes in cultured (dedifferentiated) human RPE cells and investigated the effects of C9 siRNA and C9 protein on RPE cells.

Methods

Hyperosmolarity was induced by adding 100 mM NaCl or sucrose to the culture medium. Hypoxia was induced by culturing cells in 1% O₂ or by adding the hypoxia mimetic CoCl₂. Oxidative stress was induced by adding H₂O₂

SUBMITTER: Hollborn M 

PROVIDER: S-EPMC6066273 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature