Novel hyperoxidation resistance motifs in 2-Cys peroxiredoxins.

ABSTRACT: 2-Cys peroxiredoxins (Prxs) modulate hydrogen peroxide (H₂O₂)-mediated cell signaling. At high H₂O₂ levels, eukaryotic Prxs can be inactivated by hyperoxidation and are classified as sensitive Prxs. In contrast, prokaryotic Prxs are categorized as being resistant to hyperoxidation and lack the GGLG and C-terminal YF motifs present in the sensitive Prxs. Additional molecular determinants that account for the subtle differences in the susceptibility to hyperoxidation remain to be identified. A comparison of a new, 2.15-Å-resolution crystal structure of Prx2 in the oxidized, disulfide-bonded state with the hyperoxidized structure of Prx2 and Prx1 in complex with sulfiredoxin revealed three structural regions that rearrange during catalysis. With the

SUBMITTER: Bolduc JA 

PROVIDER: S-EPMC6066324 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature