A novel Fas-binding outer membrane protein and lipopolysaccharide of Leptospira interrogans induce macrophage apoptosis through the Fas/FasL-caspase-8/-3 pathway.

ABSTRACT: Leptospira interrogans is the major causative agent of leptospirosis, an emerging, globally spreading zoonotic infectious disease. The pathogen induces macrophage apoptosis, but the molecular basis and mechanism remain unknown. In the present study, we found that L. interrogans caused apoptosis of phagocytosis-inhibited macrophages, and the product of the L. interrogans LB047 gene (Lep-OMP047) was the unique protein captured by mouse and human Fas proteins. The recombinant expressed Lep-OMP047 (rLep-OMP047) strongly bound mouse and human Fas proteins with equilibrium association constant (K_D) values of 5.20 × 10^-6 to 2.84 × 10^-9 M according to surface plasmon resonance measurement and isothermal titration calorimetry. Flow-cytometric examination showed that

SUBMITTER: Du P 

PROVIDER: S-EPMC6066479 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature