A Flow Cytometry Method for Dissecting the Cell Differentiation Process of Entamoeba Encystation.

ABSTRACT: Amoebiasis is caused by Entamoeba histolytica infection, a protozoan parasite belonging to the phylum Amoebozoa. This parasite undergoes a fundamental cell differentiation process from proliferative trophozoite to dormant cyst, termed "encystation." The cysts formed by encystation are solely responsible for the transmission of amoebiasis; therefore, Entamoeba encystation is an important subject from both biological and medical perspectives. Here, we have established a flow cytometry strategy for not only determining the percentage of formed cysts but also for monitoring changes in cell populations during encystation. This strategy together with fluorescence microscopy enables visualization of the cell differentiation process of Entamoeba encystation. We also standardiz

SUBMITTER: Mi-Ichi F 

PROVIDER: S-EPMC6066566 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature