Mycobacterium Growth Inhibition Assay of Human Alveolar Macrophages as a Correlate of Immune Protection Following Mycobacterium bovis Bacille Calmette-Guerin Vaccination.

ABSTRACT:

Background

In order to eliminate tuberculosis (TB), an effective vaccine is urgently needed to prevent infection with Mycobacterium tuberculosis. A key obstacle for the development of novel TB vaccines is the lack of surrogate markers for immune protection against M. tuberculosis.

Methods

We investigated growth rates of M. tuberculosis in the mycobacterial growth inhibition assay (MGIA) as a marker for mycobacterial growth control of human bronchoalveolar lavage (BALC) and peripheral blood mononuclear cells (PBMC) before and after vaccination with Mycobacterium bovis Bacille Calmette-Guérin (BCG) of healthy adult volunteers.

Results

Vaccination induced a positive response (p < 0.001) to purified protein derivate (PPD) in 58.8% of the

SUBMITTER: Radloff J 

PROVIDER: S-EPMC6066571 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature