Embryogenic Callus as Target for Efficient Transformation of Cyclamen persicum Enabling Gene Function Studies.

ABSTRACT: Cyclamen persicum is an ornamental plant with economic relevance in many parts of the world. Moreover, it can be regarded as an applied model for somatic embryogenesis, since transcriptomic, proteomic, and metabolomic comparisons have revealed insights into this regeneration process on the molecular level. To enable gene function analyses, the aim of this study was to establish an efficient Agrobacterium tumefaciens-mediated genetic transformation protocol for C. persicum. For the first time, embryogenic callus cultures were used as a target material. The advantages of embryogenic callus are the defined and known genotype compared to seedlings, the high regeneration potential and the stability of the regenerated plants. A. tumefaciens strains EHA105 and LBA4404

SUBMITTER: Ratjens S 

PROVIDER: S-EPMC6066641 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature