Improvement of antibody affinity by introduction of basic amino acid residues into the framework region.

ABSTRACT: Antibodies are widely used not only as therapeutic agents but also as research tools and diagnostic agents, and extensive efforts have been made to generate antibodies that have higher affinity. It was recently reported that introduction of charged residues into the framework region of an antibody improved its affinity; however, the underlying molecular mechanism has not been elucidated. In this study, we used kinetic and thermodynamic analyses of the antibody-antigen interaction to investigate the molecular mechanism by which an antibody with introduced charged residues recognizes its antigen with higher affinity. The introduction of basic amino acid residues resulted in improvement of the affinity whereas the introduction of acidic residues weakened the interaction. For two mutant antige

SUBMITTER: Fukunaga A 

PROVIDER: S-EPMC6068084 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature