Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.

ABSTRACT: Mycoplasma mobile exhibits a smooth gliding movement as does its membrane-permeabilized ghost model. Ghost experiments revealed that the energy source for M. mobile motility is adenosine triphosphate (ATP) and that the gliding comprises repetitions of 70 nm steps. Here we show a new motility mode, in which the ghost model prepared with 0.013% Triton X-100 exhibits directed rotational motions with an average speed of approximately 2.1 Hz when ATP concentration is greater than 3.0 × 10^-1 mM. We found that rotary ghosts treated with sialyllactose, the binding target for leg proteins, were stopped. Although the origin of the rotation has not been conclusively determined, this result suggested that biomolecules embedded on the cell membrane nonspecifically attach to the glass and wor

SUBMITTER: Kinosita Y 

PROVIDER: S-EPMC6068192 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature