Dataset Information

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites.

ABSTRACT: Amino-silanization of silica-based substrates has proven to be effective in guiding the immobilization of citrate-stabilized Au NPs in a good, homogeneous fashion. This accomplishment has formed the basis of fabricating micropatterns of Au NPs on such substrates by patterning of oxidized silicon wafers with (3-aminopropyl)trimethoxysilane (amino-silane) using the microcontact printing (μCP) process. This micropattern of amino-silane is used to specifically adsorb Au NPs. To avoid unspecific adsorption to the nonsilanized areas on the silicon wafers, the nonstamped areas were backfilled with self-assembled monolayers of organosilanes, for example, with methyl- or perfluoro-end-groups. Finally, after having fabricated a micropattern of Au NPs on silicon wafers, the Au NP patterns were transferred onto poly(ethylene glycol) hydrogels by our newly developed procedures, and on these nanocomposite materials, controlled cell adhesion has been achieved. Furthermore, these materials are great candidates for plasmon-based biosensor applications and also for various medical applications, such as for drug delivery systems or photothermal therapies.

SUBMITTER: Yesildag C

PROVIDER: S-EPMC6068692 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites.

Yesildag Cigdem C Bartsch Christoph C Lensen Marga C MC

ACS omega 20180703 7

Amino-silanization of silica-based substrates has proven to be effective in guiding the immobilization of citrate-stabilized Au NPs in a good, homogeneous fashion. This accomplishment has formed the basis of fabricating micropatterns of Au NPs on such substrates by patterning of oxidized silicon wafers with (3-aminopropyl)trimethoxysilane (amino-silane) using the microcontact printing (μCP) process. This micropattern of amino-silane is used to specifically adsorb Au NPs. To avoid unspecific adso ...[more]

PMID: 30087909

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Project description:Methylsulfone derivatized poly(ethylene) glycol (PEG) macromers can be biofunctionalized with thiolated ligands and cross-linked with thiol-based cross-linkers to obtain bioactive PEG hydrogels for in situ cell encapsulation. Methylsulfonyl-thiol (MS-SH) reactions present several advantages for this purpose when compared to other thiol-based cross-linking systems. They proceed with adequate and tunable kinetics for encapsulation, they reach a high conversion degree with good selectivity, and they generate stable reaction products. Our previous work demonstrated the cytocompatibility of cross-linked PEG-MS/thiol hydrogels in contact with fibroblasts. However, the cytocompatibility of the in situ MS-SH cross-linking reaction itself, which generates methylsulfinic acid as byproduct at the cross-linked site, remains to be evaluated. These studies are necessary to evaluate the potential of these systems for in vivo applications. Here we perform an extensive cytocompatibility study of PEG hydrogels during in situ cross-linking by the methylsulfonyl-thiol reaction. We compare these results with maleimide-thiol cross-linked PEGs which are well established for cell culture and in vivo experiments and do not involve the release of a byproduct. We show that fibroblasts and endothelial cells remain viable after in situ polymerization of methylsulfonyl-thiol gels on the top of the cell layers. Cell viability seems better than after in situ cross-linking hydrogels with maleimide-thiol chemistry. The endothelial cell proinflammatory phenotype is low and similar to the one obtained by the maleimide-thiol reaction. Finally, no activation of monocytes is observed. All in all, these results demonstrate that the methylsulfonyl-thiol chemistry is cytocompatible and does not trigger high pro-inflammatory responses in endothelial cells and monocytes. These results make methylsulfonyl-thiol chemistries eligible for in vivo testing and eventually clinical application in the future.

Dataset Information

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites.

Publications

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites.

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