Rapid Isolation of intact Salmonella-containing vacuoles using paramagnetic nanoparticles.

ABSTRACT:

Background

Both typhoidal and non-typhoidal Salmonella infections remain a considerable cause of morbidity and mortality globally, and impose a major socio-economic burden worldwide. A key property of all pathogenic Salmonella strains is the ability to invade host cells and reside within an intracellular, vacuolar compartment called the Salmonella-containing vacuole (SCV). Although the SCV is involved in both immune-evasion and intracellular replication and spread within the host, information about the host:pathogen interactions at this interface are limited, in part due to the technical difficulties involved in purification of these vacuoles. While a number of column- or gradient-based methods have been applied, cross-contamination with other host cell organel

SUBMITTER: Singh V 

PROVIDER: S-EPMC6069567 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature