MALDI-TOF MS monitoring of PBMC activation status in sepsis.

ABSTRACT:

Background

MALDI-TOF mass spectrometry (MS) on whole cells enables the detection of different cell types and cell activation. Here, we wondered whether this approach would be useful to investigate the host response in sepsis.

Methods

Peripheral blood mononuclear cells (PBMCs) from patients with severe sepsis and healthy donors were analyzed with MALDI-TOF MS. PBMCs from healthy donors were also stimulated with lipopolysaccharide, peptidoglycan, CpG oligonucleotides, polyinosinic polycytidylic acid, and with heat-inactivated bacteria. Averaged spectra of PBMCs stimulated in vitro by different agonists were generated from the database using the Biotyper software and matching scores between each spectrum from patients and averaged spectra from the database were calculated.

R

SUBMITTER: Daumas A 

PROVIDER: S-EPMC6069833 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature