Establishment and optimization of a liquid bead array for the simultaneous detection of ten insect-borne pathogens.

ABSTRACT:

Background

Insect-borne diseases could induce severe symptoms in human and clinical signs in animals, such as febrility, erythra, arthralgia and hemorrhagic fever, and cause significant economic losses and pose public health threat all over the world. The significant advantages of Luminex xMAP technology are high-throughput, high parallel and automation. This study aimed to establish a liquid bead array based on Luminex xMAP technology that was able to simultaneously detect multiple insect-borne pathogens.

Methods

Specific probes and primers to detect the nucleic acid of 10 insect-borne pathogens were designed. Probes were coupled with fluorescent carboxylated microspheres. The parameters of the system were optimized, including ratio of forward/reverse primers (1:2), hybridi

SUBMITTER: Wang HY 

PROVIDER: S-EPMC6069843 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature