Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining.

ABSTRACT: Hydrogen peroxide (H₂O₂) is a central reactive oxygen species (ROS) that contributes to diseases from obesity to cancer to neurodegeneration but is also emerging as an important signaling molecule. We now report a versatile histochemical approach for detection of H₂O₂ that can be employed across a broad range of cell and tissue specimens in both healthy and disease states. We have developed a first-generation H₂O₂-responsive analogue named Peroxymycin-1, which is based on the classic cell-staining molecule puromycin and enables covalent staining of biological samples and retains its signal after fixation. H₂O₂-mediated boronate cleavage uncages the puromycin aminonucleoside, which leaves a permanent and

SUBMITTER: Yik-Sham Chung C 

PROVIDER: S-EPMC6069982 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature