Cell wall-bound silicon optimizes ammonium uptake and metabolism in rice cells.

ABSTRACT:

Background and aims

Turgor-driven plant cell growth depends on cell wall structure and mechanics. Strengthening of cell walls on the basis of an association and interaction with silicon (Si) could lead to improved nutrient uptake and optimized growth and metabolism in rice (Oryza sativa). However, the structural basis and physiological mechanisms of nutrient uptake and metabolism optimization under Si assistance remain obscure.

Methods

Single-cell level biophysical measurements, including in situ non-invasive micro-testing (NMT) of NH4+ ion fluxes, atomic force microscopy (AFM) of cell walls, and electrolyte leakage and membrane potential, as well as whole-cell proteomics using isobaric tags for relative and absolute quantification (iTRAQ), were performed.

Key results

SUBMITTER: Sheng H 

PROVIDER: S-EPMC6070024 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature