Bioinformatic Expansion and Discovery of Thiopeptide Antibiotics.

ABSTRACT: Thiopeptides are members of the ribosomally synthesized and post-translationally modified peptide family of natural products. Most characterized thiopeptides display nanomolar potency toward Gram-positive bacteria by blocking protein translation with several being produced at the industrial scale for veterinary and livestock applications. Employing our custom bioinformatics program, RODEO, we expand the thiopeptide family of natural products by a factor of four. This effort revealed many new thiopeptide biosynthetic gene clusters with products predicted to be distinct from characterized thiopeptides and identified gene clusters for previously characterized molecules of unknown biosynthetic origin. To further validate our data set of predicted thiopeptide biosynthetic gene clusters, we isol

SUBMITTER: Schwalen CJ 

PROVIDER: S-EPMC6070396 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature