LED based real-time survival bioassays for nematode research.

ABSTRACT: Nematode bioassays are extensively conducted worldwide, either for screening anthelmintic drugs or for assessing the toxicity of drug candidates. Recently, the US Environmental Protection Agency mandated the use of invertebrate models including nematodes especially Caenorhabditis elegans, for toxicity testing as an alternative to rodent models. The significance of nematode bioassays in the biological sciences is escalating, but no standardized protocol is available to assess nematode mortality in a liquid medium. Manual counting under white light is the only approach currently practiced, which exhibit large variabilities and false positive results. Here, we describe an innovative counting strategy that employs light-emitting diode (LED) technology. We found that the nematodes stopped movin

SUBMITTER: Rajasekharan SK 

PROVIDER: S-EPMC6070477 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature