Transcriptional profiles of crossbred embryos derived from yak oocytes in vitro fertilized with cattle sperm.

ABSTRACT: During mammalian pre-implantation embryonic development, dramatic and orchestrated changes occur in gene transcription. Pregnancy rates were low when yak females were crossbred with cattle breeds, but few studies exist to describe the unique molecular network regulation behind the pre-implantation development of these embryos. We determined the transcriptomes of crossbred embryos derived from yak oocytes in vitro fertilized with Jersey sperm using Illumina RNA-seq for the first time in this study. Embryos were sampled at the 2-, 4-, and 8-cell, morula and blastocyst stages. The results showed that in total, 291.9 million short reads were generated from the five libraries of 2-, 4-, and 8-cell, morula and blastocyst stages, with 276.2 million high-quality reads selected for further analysis

SUBMITTER: Zi XD 

PROVIDER: S-EPMC6070518 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature