Analysis of diet-induced differential methylation, expression, and interactions of lncRNA and protein-coding genes in mouse liver.

ABSTRACT: Long non-coding RNAs (lncRNAs) regulate expression of protein-coding genes in cis through chromatin modifications including DNA methylation. Here we interrogated whether lncRNA genes may regulate transcription and methylation of their flanking or overlapping protein-coding genes in livers of mice exposed to a 12-week cholesterol-rich Western-style high fat diet (HFD) relative to a standard diet (STD). Deconvolution analysis of cell type-specific marker gene expression suggested similar hepatic cell type composition in HFD and STD livers. RNA-seq and validation by nCounter technology revealed differential expression of 14 lncRNA genes and 395 protein-coding genes enriched for functions in steroid/cholesterol synthesis, fatty acid metabolism, lipid localization, and circadian rhythm. While l

SUBMITTER: Silva JP 

PROVIDER: S-EPMC6070528 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature