Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites.

ABSTRACT: Filamentous hemagglutinin (FHA) mediates adherence and plays an important role in lower respiratory tract infections by pathogenic Bordetellae. The mature FHA proteins of B. pertussis (Bp-FHA) and the B. bronchiseptica (Bb-FHA) are generated by processing of the respective FhaB precursors by the autotransporter subtilisin-type protease SphB1. We have used bottom-up proteomics with differential ¹⁶O/¹⁸O labeling and show that despite high-sequence conservation of the corresponding FhaB segments, the mature Bp-FHA (~ 230 kDa) and Bb-FHA (~ 243 kDa) proteins are processed at different sites of FhaB, after the Ala-2348 and Lys-2479 residues, respectively. Moreover, protease surface accessibility probing by on-column (on-line) digestion of the Bp-FHA an

SUBMITTER: Jurnecka D 

PROVIDER: S-EPMC6070651 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature