Visualizing Oncolytic Virus-Host Interactions in Live Mice Using Intravital Microscopy.

ABSTRACT: Oncolytic virus (OV) therapy is an emerging cancer treatment that uses replicating viruses to infect and kill tumor cells and incite anticancer immunity. While the approach shows promise, it currently fails most patients, indicating strategies to improve OV activity are needed. Developing these will require greater understanding of OV biology, particularly in the context of OV delivery and clearance, the infection process within a complex tumor microenvironment, and the modulation of anticancer immunity. To help achieve this, we have established a technique for high-resolution 4D imaging of OV-host interactions within intact tissues of live mice using intravital microscopy (IVM). We show that oncolytic vesicular stomatitis virus (VSV) directly labeled with Alexa Fluor dyes is easily visual

SUBMITTER: Naumenko V 

PROVIDER: S-EPMC6070694 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature