Integrase-Controlled Excision of Metal-Resistance Genomic Islands in Acinetobacter baumannii.

ABSTRACT: Genomic islands (GIs) are discrete gene clusters encoding for a variety of functions including antibiotic and heavy metal resistance, some of which are tightly associated to lineages of the core genome phylogenetic tree. We have investigated the functions of two distinct integrase genes in the mobilization of two metal resistant GIs, G08 and G62, of Acinetobacter baumannii. Real-time PCR demonstrated integrase-dependent GI excision, utilizing isopropyl β-d-1-thiogalactopyranoside IPTG-inducible integrase genes in plasmid-based mini-GIs in Escherichia coli. In A. baumannii, integrase-dependent excision of the original chromosomal GIs could be observed after mitomycin C induction. In both E. coli plasmids and A. baumannii chromosome, the rate of excision an

SUBMITTER: Al-Jabri Z 

PROVIDER: S-EPMC6070778 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature