Role of Inosine⁻Uracil Base Pairs in the Canonical RNA Duplexes.

ABSTRACT: Adenosine to inosine (A⁻I) editing is the most common modification of double-stranded RNA (dsRNA). This change is mediated by adenosine deaminases acting on RNA (ADARs) enzymes with a preference of U>A>C>G for 5′ neighbor and G>C=A>U or G>C>U=A for 3′ neighbor. A⁻I editing occurs most frequently in the non-coding regions containing repetitive elements such as ALUs. It leads to disruption of RNA duplex structure, which prevents induction of innate immune response. We employed standard and biased molecular dynamics (MD) simulations to analyze the behavior of RNA duplexes with single and tandem inosine⁻uracil (I⁻U) base pairs in different sequence context. Our analysis showed that the I⁻U pairs induce changes in base pair and base pair step parameters and have different dynamics w

SUBMITTER: Spackova N 

PROVIDER: S-EPMC6070904 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature