An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana.

ABSTRACT:

Background

Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the genetic background. To achieve a systematic understanding of such GxG interactions, it is desirable to develop genome editing tools that can be rapidly deployed across many different genetic varieties.

Results

We present an efficient CRISPR/Cas9 toolbox of super module (SM) vectors. These vectors are based on a previously described fluorescence protein marker expressed in seeds allowing identification of transgene-free mutants. We have used this vector series to delete genomic regions ranging from 1.7 to 13 kb in different natural accessions of the wild plant Ar

SUBMITTER: Wu R 

PROVIDER: S-EPMC6071326 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature