Molecular characterization of Trichinella spiralis galectin and its participation in larval invasion of host's intestinal epithelial cells.

ABSTRACT: The aim of this study was to study the molecular characteristics of Trichinella spiralis galectin (Tsgal) and interactions between Tsgal and host's intestinal epithelial cells (IECs). The functional domain of Tsgal was cloned and expressed in an E. coli system. The Tsgal was 97.1% identity to the galectin of T. nativa and 20.8% identity to the galectin-8 of humans. Conserved domain analysis revealed that Tsgal belongs to TR-type galectin and has two carbon recognized domain. The rTsgal with 29.1 kDa could be recognized by T. spiralis-infected mice at 42 days post-infection (dpi). The transcription and expression of Tsgal gene was detected by RT-PCR and Western blotting in all T. spiralis developmental stages (intestinal infective larvae, adult worms, newborn larvae, and muscle larvae). The

SUBMITTER: Xu J 

PROVIDER: S-EPMC6071371 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature