Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans.

ABSTRACT: The ability to control both the means and timing of sexual reproduction provides a powerful tool to understand not only fertilization but also life history trade-offs resulting from sexual reproduction. However, precisely controlling fertilization has proved a major challenge across model systems. An ideal sterility induction system should be external, non-toxic, and reversible. Using the auxin-inducible degradation system targeting the spe-44 gene within the nematode Caenorhabditis elegans, we designed a means of externally inducing spermatogenesis arrest. We show that exposure to auxin during larval development induces both hermaphrodite self-sterility and male sterility. Moreover, male sterility can be reversed upon cessation of auxin exposure. The sterility induction syst

SUBMITTER: Kasimatis KR 

PROVIDER: S-EPMC6071612 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature