The organization of genome duplication is a critical determinant of the landscape of genome maintenance.

ABSTRACT: Genome duplication is essential for cell proliferation, and the mechanisms regulating its execution are highly conserved. These processes give rise to a spatiotemporal organization of replication initiation across the genome, referred to as the replication program. Despite the identification of such programs in diverse eukaryotic organisms, their biological importance for cellular physiology remains largely unexplored. We address this fundamental question in the context of genome maintenance, taking advantage of the inappropriate origin firing that occurs when fission yeast cells lacking the Rad3/ATR checkpoint kinase are subjected to replication stress. Using this model, we demonstrate that the replication program quantitatively dictates the extent of origin de-regulation and the clustere

SUBMITTER: Gomez-Escoda B 

PROVIDER: S-EPMC6071636 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature