Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis.

ABSTRACT: Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains incompletely understood. Previous studies have found that isolated diacylglycerol (DAG)-containing vesicles are essential for completing the fusion of the NE in nonsomatic cells. We investigated the impact of DAG depletion from the cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a mini singlet oxygen generator-C1-domain tag that localiz

SUBMITTER: Chung GHC 

PROVIDER: S-EPMC6071775 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature