Contribution of the precursors and interplay of the pathways in the phospholipid metabolism of the malaria parasite.

ABSTRACT: The malaria parasite, Plasmodium falciparum, develops and multiplies in the human erythrocyte. It needs to synthesize considerable amounts of phospholipids (PLs), principally phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS). Several metabolic pathways coexist for their de novo biosynthesis, involving a dozen enzymes. Given the importance of these PLs for the survival of the parasite, we sought to determine their sources and to understand the connections and dependencies between the multiple pathways. We used three deuterated precursors (choline-d₉, ethanolamine-d₄, and serine-d₃) to follow and quantify simultaneously their incorporations in the intermediate metabolites and the final PLs by LC/MS/MS. We show that PC

SUBMITTER: Wein S 

PROVIDER: S-EPMC6071779 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature