Structural basis for ATP-dependent chromatin remodelling by the INO80 complex.

ABSTRACT: In the eukaryotic nucleus, DNA is packaged in the form of nucleosomes, each of which comprises about 147 base pairs of DNA wrapped around a histone protein octamer. The position and histone composition of nucleosomes is governed by ATP-dependent chromatin remodellers^1-3 such as the 15-subunit INO80 complex ⁴ . INO80 regulates gene expression, DNA repair and replication by sliding nucleosomes, the exchange of histone H2A.Z with H2A, and the positioning of + 1 and -1 nucleosomes at promoter DNA^5-8. The structures and mechanisms of these remodelling reactions are currently unknown. Here we report the cryo-electron microscopy structure of the evolutionarily conserved core of the INO80 complex from the fungus Chaetomium thermophilum bound to a nucleosome, at a g

SUBMITTER: Eustermann S 

PROVIDER: S-EPMC6071913 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature