Proteomic analysis identifies transcriptional cofactors and homeobox transcription factors as TBX18 binding proteins.

ABSTRACT: The TBX18 transcription factor is a crucial developmental regulator of several organ systems in mice, and loss of its transcriptional repression activity causes dilative nephropathies in humans. The molecular complexes with which TBX18 regulates transcription are poorly understood prompting us to use an unbiased proteomic approach to search for protein interaction partners. Using overexpressed dual tagged TBX18 as bait, we identified by tandem purification and subsequent LC-MS analysis TBX18 binding proteins in 293 cells. Clustering of functional annotations of the identified proteins revealed a highly significant enrichment of transcriptional cofactors and homeobox transcription factors. Using nuclear recruitment assays as well as GST pull-downs, we validated CBFB, GAR1, IKZF2, NCOA5, SBN

SUBMITTER: Rivera-Reyes R 

PROVIDER: S-EPMC6071992 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature