Long-term stability and computational analysis of migration patterns of L-MYC immortalized neural stem cells in the brain.

ABSTRACT:

Background

Preclinical studies indicate that neural stem cells (NSCs) can limit or reverse central nervous system (CNS) damage through delivery of therapeutic agents for cell regeneration. Clinical translation of cell-based therapies raises concerns about long-term stability, differentiation and fate, and absence of tumorigenicity of these cells, as well as manufacturing time required to produce therapeutic cells in quantities sufficient for clinical use. Allogeneic NSC lines are in growing demand due to challenges inherent in using autologous stem cells, including production costs that limit availability to patients.

Methods/principal findings

We demonstrate the long-term stability of L-MYC immortalized human NSCs (LM-NSC008) cells in vivo, including engraftment, migration,

SUBMITTER: Rockne RC 

PROVIDER: S-EPMC6071994 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature