Development of an automated image analysis protocol for quantification of intracellular forms of Leishmania spp.

ABSTRACT: Leishmania parasites cause a set of neglected tropical diseases with considerable public health impact, the leishmaniases, which are often fatal if left untreated. Since current treatments for the leishmaniases exhibit high toxicity, low efficacy and prohibitive prices, many laboratories throughout the world are engaged in research for the discovery of novel chemotherapeutics. This entails the necessity of screening large numbers of compounds against the clinically relevant form of the parasite, the obligatory intracellular amastigote, a procedure that in many laboratories is still carried out by manual inspection. To overcome this well-known bottleneck in Leishmania drug development, several studies have recently attempted to automate this process. Here we implemented an image-based high

SUBMITTER: Gomes-Alves AG 

PROVIDER: S-EPMC6072083 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature