Colonization efficiency of Pseudomonas putida is influenced by Fis-controlled transcription of nuoA-N operon.

ABSTRACT: Root colonization of plant growth-promoting bacteria is a complex multistep process that is influenced by several factors. For example, during adherence to plant roots, bacteria have to endure reactive oxygen species (ROS) produced by plants. In this study, we report that the global transcriptional regulator Fis is involved in the regulation of ROS-tolerance of Pseudomonas putida and thereby affects barley root colonization. Fis overexpression reduced both ROS-tolerance and adherence to barley roots and activated the transcription of the nuoA-N operon encoding NADH dehydrogenase I, the first enzyme of a membrane-bound electron-transport chain. The nuoA-N knockout mutation in the fis-overexpression background increased the ROS-tolerance and adherence to barley roots. We show that nuoA has t

SUBMITTER: Teppo A 

PROVIDER: S-EPMC6072106 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature