Quantitative Proteome and Phosphoproteome Analyses of <i>Streptomyces coelicolor</i> Reveal Proteins and Phosphoproteins Modulating Differentiation and Secondary Metabolism.

ABSTRACT: Streptomycetes are multicellular bacteria with complex developmental cycles. They are of biotechnological importance as they produce most bioactive compounds used in biomedicine, e.g. antibiotic, antitumoral and immunosupressor compounds. Streptomyces genomes encode many Ser/Thr/Tyr kinases, making this genus an outstanding model for the study of bacterial protein phosphorylation events. We used mass spectrometry based quantitative proteomics and phosphoproteomics to characterize bacterial differentiation and activation of secondary metabolism of Streptomyces coelicolor We identified and quantified 3461 proteins corresponding to 44.3% of the S. coelicolor proteome across three developmental stages: vegetative hypha (first mycelium); secondary metabolite producin

SUBMITTER: Rioseras B 

PROVIDER: S-EPMC6072539 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature