Integrative Genome-Wide Analysis of Long Noncoding RNAs in Diverse Immune Cell Types of Melanoma Patients.

ABSTRACT: Genome-wide identification and characterization of long noncoding RNAs (lncRNA) in individual immune cell lineages helps us better understand the driving mechanisms behind melanoma and advance personalized patient treatment. To elucidate the transcriptional landscape in diverse immune cell types of peripheral blood cells (PBC) in stage IV melanoma, we used whole transcriptome RNA sequencing to profile lncRNAs in CD4⁺, CD8⁺, and CD14⁺ PBC from 132 patient samples. Our integrative computational approach identified 27,625 expressed lncRNAs, 2,744 of which were novel. Both T cells (i.e., CD4⁺ and CD8⁺ PBC) and monocytes (i.e., CD14⁺ PBC) exhibited differential transcriptional expression profiles between patients with melanoma an

SUBMITTER: Wang L 

PROVIDER: S-EPMC6072578 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature